Molecular Microscopy of Membranes

Daniel Levy

Daniel Lévy Team Leader Tel:

aurelie_bertin

Aurelie Bertin Project leader Tel:

How the components of cell membranes, lipids, transmembrane proteins, and bound cytoskeleton proteins, are organized and how the interplay between their molecular 3D architecture and the surrounding membrane affects their function? Our Team addresses these general questions in the context of cell division, cell detoxification and inter-organelles communication. We combine cryo-electron microscopy, single particle analysis, cryo-tomography and novel in vitro membrane systems to decipher the architectures of both proteins and membrane. We integrate our results with data derived from functional analysis, physics of membranes and cell biology to unravel mechanisms at different spatial and temporal scales.

web-1MMM

From left to right: Energized transport of drugs by an ABC transporter reconstituted in GUV (Dezi et al., PNAS 2013). Cryo-EM of Myosin 1b specifically bound to Pip2 vesicle (Yamada et al., Nat Comm. 2014). Cryo-tomography of septin filaments polymerized on Pip2 vesicles. 3D reconstruction of an ABC transporter embedded in a lipid bilayer (Fribourg, J.Mol. Biol. 2014)

Key publications

Year of publication 2017

P Guichard, V Hamel, M Le Guennec, N Banterle, I Iacovache, V Nemčíková, I Flückiger, K N Goldie, H Stahlberg, D Lévy, B Zuber, P Gönczy (2017 Mar 24)

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms.

Nature communications : 14813 : DOI : 10.1038/ncomms14813

Year of publication 2014

Guillaume van Niel, Ptissam Bergam, Aurelie Di Cicco, Ilse Hurbain, Alessandra Lo Cicero, Florent Dingli, Roberta Palmulli, Cecile Fort, Marie Claude Potier, Leon J Schurgers, Damarys Loew, Daniel Levy, Graça Raposo (2014 Nov 13)

Apolipoprotein E Regulates Amyloid Formation within Endosomes of Pigment Cells.

Cell reports : 43-51 : DOI : 10.1016/j.celrep.2015.08.057
Ayako Yamada, Alexandre Mamane, Jonathan Lee-Tin-Wah, Aurélie Di Cicco, Coline Prévost, Daniel Lévy, Jean-François Joanny, Evelyne Coudrier, Patricia Bassereau (2014 Apr 7)

Catch-bond behaviour facilitates membrane tubulation by non-processive myosin 1b.

Nature communications : 3624 : DOI : 10.1038/ncomms4624

Year of publication 2013

Pierre Frederic Fribourg, Mohamed Chami, Carlos Oscar S Sorzano, Francesca Gubellini, Roberto Marabini, Sergio Marco, Jean-Michel Jault, Daniel Lévy (2013 Aug 7)

3D cryo-electron reconstruction of BmrA, a bacterial multidrug ABC transporter in an inward-facing conformation and in a lipidic environment.

Journal of molecular biology : 2059-69 : DOI : 10.1016/j.jmb.2014.03.002
Manos Mavrakis, Yannick Azou-Gros, Feng-Ching Tsai, José Alvarado, Aurélie Bertin, Francois Iv, Alla Kress, Sophie Brasselet, Gijsje H Koenderink, Thomas Lecuit (2013 Jun 10)

Septins promote F-actin ring formation by crosslinking actin filaments into curved bundles.

Nature cell biology : 322-34 : DOI : 10.1038/ncb2921
Manuela Dezi, Aurelie Di Cicco, Patricia Bassereau, Daniel Lévy (2013 Apr 15)

Detergent-mediated incorporation of transmembrane proteins in giant unilamellar vesicles with controlled physiological contents.

Proceedings of the National Academy of Sciences of the United States of America : 7276-81 : DOI : 10.1073/pnas.1303857110
All publications