Molecular Microscopy of Membranes

Daniel Levy

Daniel Lévy Team Leader Tel:

aurelie_bertin

Aurelie Bertin Project leader Tel:

11-14jpegHow the components of cell membranes, lipids, transmembrane proteins, and bound cytoskeleton proteins, are organized and how the interplay between their molecular 3D architecture and the surrounding membrane affects their function? Our Team addresses these general questions in the context of cell division, cell detoxification and inter-organelles communication. We combine cryo-electron microscopy, single particle analysis, cryo-tomography and novel in vitro membrane systems to decipher the architectures of both proteins and membrane. We integrate our results with data derived from functional analysis, physics of membranes and cell biology to unravel mechanisms at different spatial and temporal scales.

web-1MMM

From left to right: Energized transport of drugs by an ABC transporter reconstituted in GUV (Dezi et al., PNAS 2013). Cryo-EM of Myosin 1b specifically bound to Pip2 vesicle (Yamada et al., Nat Comm. 2014). Cryo-tomography of septin filaments polymerized on Pip2 vesicles. 3D reconstruction of an ABC transporter embedded in a lipid bilayer (Fribourg, J.Mol. Biol. 2014)

Key publications

Year of publication 2020

Aurélie Bertin , Nicola de Franceschi , Eugenio de la Mora , Sourav Maiti, Maryam Alqabandi, Nolwen Miguet, Aurélie di Cicco, Wouter H. Roos, Stéphanie Mangenot , Winfried Weissenhorn, Patricia Bassereau (2020 May 29)

Human ESCRT-III polymers assemble on positively curved membranes and induce helical membrane tube formation

Nature Communications : 11 : 2663 : DOI : 10.1038/s41467-020-16368-5

Year of publication 2019

Jamecna D, Polidori DJ, Mesmin B, Dezi M, Lévy D, Bigay J, Antonny B (2019 Mar 22)

An intrinsically disordered region in OSBP acts as an entropic barrier to control protein dynamics and orientation at membrane contact sites

Developmental cell * : * highlighted Trend in Cell Biology 2019 : DOI : 10.1016/j.devcel.2019.02.021
Simon C*, Kusters R*, Caorsi V*, Allard A, Abou-Ghali M, Manzi J, Di Cicco A, Lévy D, Lenz M, Joanny J-F, Campillo C, Plastino J, Sens P*, Sykes C* (2019 Mar 18)

Actin dynamics drive cell-like membrane deformation

Nature Physics : DOI : 10.1038/s41567-019-0464-1
Beber A, Taveneau C, Nania M, Tsai FC, Di Cicco A, Bassereau P, Lévy D, Cabral JT, Isambert H, Mangenot S*, Bertin A* (2019 Jan 24)

Membrane reshaping by micrometric curvature sensitive septin filaments

Nature communications : DOI : 10.1038/s41467-019-08344-5

Year of publication 2018

Feng-Ching Tsai*, Aurelie Bertin*, Hugo Bousquet, John Manzi, Yosuke Senju, Meng-Chen Tsai, Laura Picas, Stephanie Miserey-Lenkei, Pekka Lappalainen, Emmanuel Lemichez, Evelyne Coudrier*, Patricia Bassereau* (2018 Sep 30)

Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner.

elife : 7 : e37262 : DOI : 10.7554/eLife.37262

Year of publication 2017

P Guichard, V Hamel, M Le Guennec, N Banterle, I Iacovache, V Nemčíková, I Flückiger, K N Goldie, H Stahlberg, D Lévy, B Zuber, P Gönczy (2017 Mar 24)

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms.

Nature communications : 14813 : DOI : 10.1038/ncomms14813
All publications