Year of publication 2019
Meeting report – Building the Cell 2018journal of cell science : 132 : DOI : 10.1242/jcs.229765 Learn more
Cell biologists from all around the world gathered in Paris on the 26 to 28 September 2018 to participate in the 3rd international meeting ‘Building the Cell’. It was organized by Hélène Barelli, Arnaud Echard, Thierry Galli, Florence Niedergang, Manuel Théry and Marie Hélène Verlhac on behalf of the French Society for Cell Biology (SBCF) at the Institut Pasteur. Around 230 participants joined the meeting for stimulating talks, discussions, poster sessions, and a gala dinner on the Seine that included a music performance by the rock group ‘Membrane Band’. The unifying theme of the meeting was the development of creative multidisciplinary approaches to understand cellular life at different scales in a dynamic and quantitative manner. Here, we summarize the results presented at the meeting and the emerging ideas from the different sessions.
The 3rd international meeting ‘Building the Cell’ (Fig. 1; Fig. 2) was divided into ten different sessions that covered a variety of topics including intracellular trafficking, cell division, cytoskeletal dynamics and cell mechanics, cancer and stem cell biology, embryonic development and tissue morphogenesis, neurobiology, and aggregates and phase transitions. A broad spectrum of modern approaches and experimental systems ranging from synthetic biology and stem cell technologies to 3D organoids and animal models was presented. The meeting highlighted some of the latest and novel findings in cell biology, often coupled to major methodological developments in quantitative microscopy and computational modelling, as well as cell and tissue micro-engineering.
Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivoNature Communications : 10 : 556 : DOI : 10.1038/s41467-019-08442-4 Learn more
Adaptive F-actin polymerization and localized ATP production drive basement membrane invasion in the absence of MMPsDevelopmental Cell : 48 : 313-328 : DOI : 10.1016/j.devcel.2018.12.018 Learn more
Actin dynamics drive cell-like membrane deformation.Nature Physics : Accepted Learn more
Cell membrane deformations are crucial for proper cell function. Specialized protein assemblies initiate inward or outward membrane deformations that turn into, for example, endocytic intermediates or filopodia. Actin assembly and dynamics are involved in this process, although their detailed role remains controversial. We show here that a dynamic, branched actin network is sufficient to initiate both inward and outward membrane deformation. With actin polymerization triggered at the membrane of liposomes, we produce inward filopodia-like structures at low tension, while outward endocytosis-like structures are robustly generated regardless of tension. Our results shed light on the mechanism of endocytosis, both in mammalian cells, where actin polymerization forces are required when membrane tension is increased, and in yeast, where those forces are necessary to overcome the opposing turgor pressure. By combining experimental observations with physical modeling, we propose a mechanism for actin-driven endocytosis controlled by membrane tension and the architecture of the actin network.Fold up