Biochemistry, Molecular Biology and Cells Platform

BMBC168 is a shared platform for biochemistry, molecular biology  and cell biology techniques in our department.

• Submit your project to the following address  and put your group leader/project leader in copy.

What we know how to do for you (or can help you do yourself): 

Molecular Biology:

• Molecular cloning (digestion/ligation, InFusion ligation, Gateway cloning)
• Sequencing
• DNA purification (Maxi prep etc)
• PCR (DNA amplification)
• Mutagenesis
• Genomic DNA preparation
• qPCR (quantitative PCR)
• Microarrays
• FISH
• constructions for CRISPR

Protein Purification:

• Protein expression systems: E. coli, yeast (S. cerevisiae), baculovirus (in insect cells–Sf9), mammalian cells (Flp-In system HEK293 cells), cell-free protein expression
• Protein purification from tissues (brain, muscle…)
• Affinity chromatography using tags (His, GST etc)
• FPLC purification with or without tags
• Mass spectrometry analysis of proteins
• Purification of membrane proteins
• Basic techniques: SDS-PAGE gels, native gels, protein concentration by Bradford, protein labeling with fluorescent markers (Alexa etc)

Cell Biology Techniques:

• Transient transfection
• Stable cell line production (electroporation, lipofectants reagents, lentiviral/retroviral infection)
• Lentiviral production, concentration, titration, storage
• Immunofluorescence
• Confocal microscopy
• Flow cytometry (analyse and cell sorting)
• CRISPR
• Mycoplasma test
• ELISA test (checking absence viruses)
• STR authentication
• Microfabrication