Biochemistry, Molecular Biology and Cells Platform

BMBC168 is a shared platform for biochemistry, molecular biology  and cell biology techniques in our department.

  • Submit your project to the following address  and put your group leader/project leader in copy.

What we know how to do for you (or can help you do yourself): 

Molecular Biology:

  • Molecular cloning (digestion/ligation, InFusion ligation, Gateway cloning)
  • Sequencing
  • DNA purification (Maxi prep etc)
  • PCR (DNA amplification)
  • Mutagenesis
  • Genomic DNA preparation
  • qPCR (quantitative PCR)
  • Microarrays
  • FISH
  • constructions for CRISPR

Protein Purification:

  • Protein expression systems: E. coli, yeast (S. cerevisiae), baculovirus (in insect cells–Sf9), mammalian cells (Flp-In system HEK293 cells), cell-free protein expression
  • Protein purification from tissues (brain, muscle…)
  • Affinity chromatography using tags (His, GST etc)
  • FPLC purification with or without tags
  • Mass spectrometry analysis of proteins
  • Purification of membrane proteins
  • Basic techniques: SDS-PAGE gels, native gels, protein concentration by Bradford, protein labeling with fluorescent markers (Alexa etc)

Cell Biology Techniques:

  • Transient transfection
  • Stable cell line production (electroporation, lipofectants reagents, lentiviral/retroviral infection)
  • Lentiviral production, concentration, titration, storage
  • Immunofluorescence
  • Confocal microscopy
  • Flow cytometry (analyse and cell sorting)
  • CRISPR
  • Mycoplasma test
  • ELISA test (checking absence viruses)
  • STR authentication
  • Microfabrication

 

Key publications

Year of publication 2017

Chaput Ludovic, Mouawad Liliane (2017 Jun 12)

Efficient conformational sampling and weak scoring in docking programs? Strategy of the wisdom of crowds

Journal of Cheminformatics : 9 : 37 : DOI : 10.1186/s13321-017-0227-x

Year of publication 2013

Souibgui A., Gaucher A., Marrot J., Aloui F., Mahuteau-Betzer F., Ben Hassine B., Prim D. (2013 Jul 1)

A Flexible Strategy Towards Thienyl-, Oxazolyl- and Pyridyl-Fused Fluorenones

European Journal of Organic Chemistry : 2013 : 4515-4522 : DOI : 10.1002/ejoc.201300233