Translational Immunotherapy

Publications

Year of publication 2011

Maher M Almubarak, Marick Laé, Wulfran Cacheux, Patricia de Cremoux, Jean-Yves Pierga, Fabien Reyal, Simon P Bennett, Marie-Christine Falcou, Remy J Salmon, Bernard Baranger, Pascale Mariani (2011 May 28)

Gastric metastasis of breast cancer: a single centre retrospective study.

Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver : 823-7 : DOI : 10.1016/j.dld.2011.04.009 Learn more
Summary

Digestive metastasis of breast cancer are rare but when they do occur the stomach is one of the commoner sites.

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Sophie Haberer, Lisa Belin, Romuald Le Scodan, Youlia M Kirova, Alexia Savignoni, Denise Stevens, Patricia Moisson, Didier Decaudin, Jean-Yves Pierga, Fabien Reyal, François Campana, Alain Fourquet, Marc A Bollet (2011 May 25)

Locoregional treatment for breast carcinoma after Hodgkin’s lymphoma: the breast conservation option.

International journal of radiation oncology, biology, physics : e145-52 : DOI : 10.1016/j.ijrobp.2011.03.013 Learn more
Summary

To report clinical and pathologic characteristics and outcome of breast cancer (BC) after irradiation for Hodgkin’s lymphoma (HL) in women treated at the Institut Curie, with a special focus on the breast-conserving option.

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Camille Sabbah, Gildas Mazo, Caroline Paccard, Fabien Reyal, Philippe Hupé (2011 Apr 16)

SMETHILLIUM: spatial normalization method for Illumina infinium HumanMethylation BeadChip.

Bioinformatics (Oxford, England) : 1693-5 : DOI : 10.1093/bioinformatics/btr187 Learn more
Summary

DNA methylation is a major epigenetic modification in human cells. Illumina HumanMethylation27 BeadChip makes it possible to quantify the methylation state of 27 578 loci spanning 14 495 genes. We developed a non-parametric normalization method to correct the spatial background noise in order to improve the signal-to-noise ratio. The prediction performance of the proposed method was assessed on three fully methylated samples and three fully unmethylated DNA samples. We demonstrate that the spatial normalization outperforms BeadStudio to predict the methylation state of a given locus.

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Youlia M Kirova, Vincent Servois, Fabien Reyal, Dominique Peurien, Alain Fourquet, Nathalie Fournier-Bidoz (2011 Mar 1)

Use of deformable image fusion to allow better definition of tumor bed boost volume after oncoplastic breast surgery.

Surgical oncology : e123-5 : DOI : 10.1016/j.suronc.2011.02.001 Learn more
Summary

Although the use of boost irradiation is recommended, the standard technique and definition of the boost volume after oncoplastic surgery have not been clearly established. This multidisciplinary study based on image registration was designed to propose practical solutions for the definition of tumor bed boost in this setting.

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Eugenie Guillot, Benoit Couturaud, Fabien Reyal, Alain Curnier, Julie Ravinet, Marick Laé, Marc Bollet, Jean-Yves Pierga, Remy Salmon, Alfred Fitoussi, (2011 Jan 22)

Management of phyllodes breast tumors.

The breast journal : 129-37 : DOI : 10.1111/j.1524-4741.2010.01045.x Learn more
Summary

Phyllodes tumors are a rare distinctive fibroepithelial tumors of the breast and their management continues to be questioned. The aim of our study was to examine the treatment and outcome of 165 patients with phyllodes tumors and to review the options for surgical management. This is a retrospective study of 165 patients who presented to the Institut Curie between January 1994 and November 2008 for benign, borderline or malignant phyllodes tumors. The median follow-up was 12.65 months [range 0-149.8]. The median age at diagnosis was 44 years [range 17-79]. One hundred and sixty patients (97%) had breast-conserving treatment, of whom 3 patients (1.8%) had oncoplastic breast surgery. Younger women had a significantly higher chance of having a benign phyllodes tumor (p = 0.0001) or a tumor of small size (p < 0.0001). Histologic examination showed 114 benign (69%), 37 borderline (22%) and 14 malignant tumors (9%). The median tumor size was 30 mm [range 5-150]. The tumor margins were considered incomplete (< 10 mm) in 46 out of 165 cases (28%) with 52% revision surgery. Only the tumor grade was a significant risk factor for incomplete tumor margins (p = 0.005). Fifteen patients developed local recurrence (10%) and two, metastases. In univariate analysis, the histologic grade (p = 0.008), and tumor size (p = 0.02) were significative risk factors for local recurrence with an accentuated risk for "borderline" tumors and tumors of large size.).Similar results were obtained using multivariate analysis (p = 0.07). The mainstay of treatment for phyllodes tumors remains excision with a safe surgical margin, taking advantage breast conserving surgery where amenable. For borderline or malignant phyllodes tumors or in cases of local tumor recurrence, mastectomy, and immediate breast reconstruction may become the preferred option. Genetic analysis will potentially supplement classical histologic examination in order to improve our management of these tumors. The role of adjuvant treatments is unproven and must be considered on a case-by-case basis.

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Year of publication 2010

Marc A Bollet, Youlia M Kirova, Alain Fourquet, Patricia de Cremoux, Fabien Reyal (2010 Aug 26)

Prognostic factors for local recurrence following breast-conserving treatment in young women.

Expert review of anticancer therapy : 1215-27 : DOI : 10.1586/era.10.67 Learn more
Summary

Breast cancer is a rare disease in young women. Its incidence has remained remarkably constant over the last decades, contrary to what has happened in older patients. Age is a major prognostic factor for local control after breast-conserving treatments, which does not seem to be entirely explained by the poor prognostic features that are more commonly associated with breast cancers in young women. A better knowledge of the prognostic factors for local control, and, whenever possible, those pertaining specifically to the young age group, is key, not only to better select the patients who would benefit the most from a breast-conserving approach but also to enhance the local control when this option has been elected. These prognostic factors can be related to the patient, the tumor and the treatment they receive. Finally, we will present a perspective of what the future could hold, both in terms of the selection of the patients and of a more customized therapeutic approach.

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Caroline Daveau, Alexia Savignoni, Soumya Abrous-Anane, Jean-Yves Pierga, Fabien Reyal, Chantal Gautier, Youlia M Kirova, Rémi Dendale, François Campana, Alain Fourquet, Marc A Bollet (2010 Jul 8)

Is radiotherapy an option for early breast cancers with complete clinical response after neoadjuvant chemotherapy?

International journal of radiation oncology, biology, physics : 1452-9 : DOI : 10.1016/j.ijrobp.2010.01.003 Learn more
Summary

To determine whether the exclusive use of radiotherapy (ERT) could be a treatment option after complete clinical response (cCR) to neoadjuvant chemotherapy (NCT) for early breast cancer (EBC).

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Soumya Abrous-Anane, Alexia Savignoni, Caroline Daveau, Jean-Yves Pierga, Chantal Gautier, Fabien Reyal, Remi Dendale, François Campana, Youlia M Kirova, Alain Fourquet, Marc A Bollet (2010 May 19)

Management of inflammatory breast cancer after neoadjuvant chemotherapy.

International journal of radiation oncology, biology, physics : 1055-63 : DOI : 10.1016/j.ijrobp.2009.12.009 Learn more
Summary

To assess the benefit of breast surgery for inflammatory breast cancer (IBC).

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Alfred D Fitoussi, M G Berry, Fausto Famà, Marie-Christine Falcou, Alain Curnier, Benoit Couturaud, Fabien Reyal, Remy J Salmon (2010 Feb 4)

Oncoplastic breast surgery for cancer: analysis of 540 consecutive cases [outcomes article].

Plastic and reconstructive surgery : 454-62 : DOI : 10.1097/PRS.0b013e3181c82d3e Learn more
Summary

Synchronous plastic and oncological surgery is undertaken to improve the security of excision margins and yield high-quality aesthetic outcomes when conventional breast-conserving therapy either anticipates poor results or is not possible.

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Year of publication 2009

Patricia De Cremoux, Laure Grandin, Véronique Diéras, Alexia Savignoni, Armelle Degeorges, Rémy Salmon, Marc A Bollet, Fabien Reyal, Brigitte Sigal-Zafrani, Anne Vincent-Salomon, Xavier Sastre-Garau, Henri Magdelénat, Laurent Mignot, Alain Fourquet, (2009 May 16)

Urokinase-type plasminogen activator and plasminogen-activator-inhibitor type 1 predict metastases in good prognosis breast cancer patients.

Anticancer research : 1475-82 Learn more
Summary

This retrospective analysis was designed to confirm the predictive role of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor type I (PAI-1) in the outcome of early stage, node-negative breast cancer patients.

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Year of publication 2004

Krishna B Clough, Sunil S Thomas, Alfred D Fitoussi, Benoit Couturaud, Fabien Reyal, Marie-Christine Falcou (2004 Dec 4)

Reconstruction after conservative treatment for breast cancer: cosmetic sequelae classification revisited.

Plastic and reconstructive surgery : 1743-53 Learn more
Summary

Patients with suboptimal results following breast conservative therapy are presenting more frequently to plastic surgeons as a difficult management problem. A three-type “cosmetic sequelae classification” is proposed to evaluate and manage these patients. From February of 1991 to November of 2001, 85 patients were treated for cosmetic sequelae of breast conservative therapy at the Institut Curie. The patients were followed up prospectively for 6 to 132 months (median, 33 months). They were assessed with regard to age, site and stage of tumor, type of initial breast conservative therapy undertaken, corrective operative procedures performed, complications, and cosmetic results. Forty-eight patients (56.5 percent) had type 1 cosmetic sequelae, 33 patients (38.8 percent) had type 2, and four patients (4.7 percent) had type 3. Type 1 was managed by contralateral symmetrizing procedures. Type 2 was the most difficult to manage by means of various procedures. Type 3 required mastectomy and immediate breast reconstruction. Type 1 had 97.6 percent good results compared with 82.7 percent for type 2. Three of the four type 3 patients had good results. This article reaffirms the validity of the cosmetic sequelae classification as a simple, practical guide for breast reconstructive surgeons. It discusses the various choices of reconstructive procedures available, the importance of preventing these cosmetic sequelae, and the role of the plastic surgeon in the planning of conservative treatment of breast cancers.

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Year of publication 1983

W J Hadlow, R E Race, R C Kennedy (1983 Sep 1)

Comparative pathogenicity of four strains of Aleutian disease virus for pastel and sapphire mink.

Infection and immunity : 1016-23 Learn more
Summary

Information was sought on the comparative pathogenicity of four North American strains (isolates) of Aleutian disease virus for royal pastel (a non-Aleutian genotype) and sapphire (an Aleutian genotype) mink. The four strains (Utah-1, Ontario [Canada], Montana, and Pullman [Washington]), all of mink origin, were inoculated intraperitoneally and intranasally in serial 10-fold dilutions. As indicated by the appearance of specific antibody (counterimmunoelectrophoresis test), all strains readily infected both color phases of mink, and all strains were equally pathogenic for sapphire mink. Not all strains, however, regularly caused Aleutian disease in pastel mink. Infection of pastel mink with the Utah-1 strain invariably led to fatal disease. Infection with the Ontario strain caused fatal disease nearly as often. The Pullman strain, by contrast, almost never caused disease in infected pastel mink. The pathogenicity of the Montana strain for this color phase was between these extremes. These findings emphasize the need to distinguish between infection and disease when mink are exposed to Aleutian disease virus. The distinction has important implications for understanding the natural history of Aleutian disease virus infection in ranch mink.

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Year of publication 1982

T W Doucet, J J Hurwitz (1982 Feb 1)

Canaliculodacryocystorhinostomy in the treatment of canalicular obstruction.

Archives of ophthalmology (Chicago, Ill. : 1960) : 306-9 Learn more
Summary

Epiphora secondary to canalicular obstruction has been a perplexing problem most often treated with Jones’ tube bypass surgery, which necessitates a permanent prosthesis and long-term follow-up. There are certain cases in which these problems may be avoided by using an alternative surgical approach. The canaliculodacryocystorhinostomy, a microsurgery technique, reconstructs rather than bypasses the physiological system. We recommend this procedure in all cases of lateral common canalicular obstruction and upper and lower canalicular obstruction with greater than 8 mm of patency.

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Year of publication 1981

L M Cruz-Orive, E R Weibel (1981 Jun 1)

Sampling designs for stereology.

Journal of microscopy : 235-57 Learn more
Summary

The purpose of this paper is to propose the necessary sampling techniques for estimating a global parameter defined in a solid opaque specimen (e.g. the total volume of mitochondria in a given liver, the total capillary surface area in a given lung, etc). The geometry of the specimen often suggests a multi-level or cascade sampling design at different magnifications, whereby the object phase at one level becomes the reference phase in the next level. The final parameter is then estimated as the product of the intermediate ratios with the volume of the specimen, which is estimated independently. Each level can be regarded as an independent sampling design; a given stereological project may be planned in terms of one or more of these designs. Our development is a blend of practical experience and recent theoretical advances on sampling for stereology with well-known sampling techniques previously developed with different purposes in mind.

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Year of publication 1980

P Youderian, M M Susskind (1980 Nov 1)

Identification of the products of bacteriophage P22 genes, including a new late gene.

Virology : 258-69 Learn more
Summary

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