Modeling and Multimodal and Multiparametric Imaging

Our goal is to contribute, via integrated-imaging approaches and molecular modelling, to drug design and to the understanding of the effects and action mechanisms of diagnostic, therapeutic, and theranostic agents on cells, tissues and organisms.

 

Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).
Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).

For this purpose, we develop and optimize methods and tools (software, image acquisition procedures, preparation techniques and methods to improve sensitivity and resolution of imaging equipment) in the context of multimodal and/or multiscale imaging (including nano-SIMS, TEM-STEM-EFTEM, MRI) and molecular modelling (docking and dynamics). We mainly apply these developments to oncology and radio-biology thru close collaborations with several biology groups. We focus our activities on:

  1. the multiparametric/multiscale imaging developments to assess heterogeneity of healthy and cancer tissues characteristics
  2. the understanding of the effects of photon irradiation of photosensitizing molecules
  3. the characterization and tracking of radiosensitizing nanoparticles and drugs within cells
  4. the study of the bio-distribution and pharmacokinetic of drugs and chemical elements (I, P, Ca, Fe,….) in normal or pathogenic tissues and cells
  5. the search of small molecules with medicinal interest by computer-aided drug design
  6. the understanding at the atomic level of the mechanism of action of some proteins of biological interest by the use of normal mode analysis (NMA) and molecular dynamics (MD).

The developments and tools we design becomes accessible to academic users and corporations through the facilities depending on us (MRI, chemical mapping and tomography as part of the Cellular and Imaging facility of the Institut Curie) and through the Institut Carnot-Global Care.

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Key publications

Year of publication 2019

Ouahiba Sihali-Beloui, Djamila Aroune, Fella Benazouz, Adile Hadji, Salima El-Aoufi, Sergio Marco (2019 Jun 2)

A hypercaloric diet induces hepatic oxidative stress, infiltration of lymphocytes, and mitochondrial reshuffle in Psammomys obesus, a murine model of insulin resistance.

Comptes rendus biologies : DOI : S1631-0691(19)30041-1
Dapeng Zhang, Yujiao Fan, Hui Chen, Sylvain Trépout, Min-Hui Li (2019 May 31)

CO2-activated reversible transition between polymersomes and micelles with AIE fluorescence.

Angewandte Chemie (International ed. in English) : Accepted Author Manuscript : DOI : 10.1002/anie.201905089
El Hassen Mokrani, Abderrahmane Bensegueni, Ludovic Chaput, Claire Beauvineau, Hanane Djeghim, Liliane Mouawad (2019 May 1)

Identification of New Potent Acetylcholinesterase Inhibitors Using Virtual Screening and In Vitro Approaches.

Molecular informatics : 38 : 1800118 : DOI : 10.1002/minf.201800118
David Partouche, Jérémie Mathurin, Antoine Malabirade, Sergio Marco, Christophe Sandt, Véronique Arluison, Ariane Deniset-Besseau, Sylvain Trépout (2019 Apr 1)

Correlative infrared nanospectroscopy and transmission electron microscopy to investigate nanometric amyloid fibrils: prospects and challenges.

Journal of microscopy : 274 : 23-31 : DOI : 10.1111/jmi.12779
Nathalie Fretellier, Agnès Granottier, Marlène Rasschaert, Anne-Laure Grindel, Fannie Baudimont, Philippe Robert, Jean-Marc Idée, Claire Corot (2019 Feb 1)

Does Age Interfere With Gadolinium Toxicity and Presence in Brain and Bone Tissues?: A Comparative Gadoterate Versus Gadodiamide Study in Juvenile and Adult Rats.

Investigative radiology : 54 : 61-71 : DOI : 10.1097/RLI.0000000000000517
M Schmidt-Cernohorska, I Zhernov, E Steib, M Le Guennec, R Achek, S Borgers, D Demurtas, L Mouawad, Z Lansky, V Hamel, P Guichard (2019 Jan 19)

Flagellar microtubule doublet assembly in vitro reveals a regulatory role of tubulin C-terminal tails.

Science (New York, N.Y.) : 363 : 285-288 : DOI : 10.1126/science.aav2567
All publications