Modeling and Multimodal and Multiparametric Imaging

Sergio Marco

Sergio Marco Team Leader

Our goal is to contribute, via integrated-imaging approaches and molecular modelling, to drug design and to the understanding of the effects and action mechanisms of diagnostic, therapeutic, and theranostic agents on cells, tissues and organisms.

 

Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).
Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).

For this purpose, we develop and optimize methods and tools (software, image acquisition procedures, preparation techniques and methods to improve sensitivity and resolution of imaging equipment) in the context of multimodal and/or multiscale imaging (including nano-SIMS, TEM-STEM-EFTEM, MRI) and molecular modelling (docking and dynamics). We mainly apply these developments to oncology and radio-biology thru close collaborations with several biology groups. We focus our activities on:

  1. the multiparametric/multiscale imaging developments to assess heterogeneity of healthy and cancer tissues characteristics
  2. the understanding of the effects of photon irradiation of photosensitizing molecules
  3. the characterization and tracking of radiosensitizing nanoparticles and drugs within cells
  4. the study of the bio-distribution and pharmacokinetic of drugs and chemical elements (I, P, Ca, Fe,….) in normal or pathogenic tissues and cells
  5. the search of small molecules with medicinal interest by computer-aided drug design
  6. the understanding at the atomic level of the mechanism of action of some proteins of biological interest by the use of normal mode analysis (NMA) and molecular dynamics (MD).

The developments and tools we design becomes accessible to academic users and corporations through the facilities depending on us (MRI, chemical mapping and tomography as part of the Cellular and Imaging facility of the Institut Curie) and through the Institut Carnot-Global Care.

Marco2

Key publications

Year of publication 2017

Slodzian G., Wu T.D., Duprat J., Engrand C., Guerquin-Kern J.L. (2017 Dec 1)

Dynamic transfer applied to secondary ion imaging over large scanned fields with the nanoSIMS 50 at high mass resolution

Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms : 412 : 123-173 : DOI : 10.1016/j.nimb.2017.06.019
Antoine Malabirade, Javier Morgado-Brajones, Sylvain Trépout, Frank Wien, Ileana Marquez, Jérôme Seguin, Sergio Marco, Marisela Velez, Véronique Arluison (2017 Sep 8)

Membrane association of the bacterial riboregulator Hfq and functional perspectives.

Scientific reports : 7 : 10724 : DOI : 10.1038/s41598-017-11157-5
Sylvain Trépout, Anne Marie Wehenkel (2017 Aug 31)

Bacterial Tubulins: A Eukaryotic-Like Microtubule Cytoskeleton

Trends in Microbiology : In press, corrected proof : DOI : 10.1016/j.tim.2017.08.004
Donati L., Nilchian M., Trepout S., Messaoudi C., Marco S., Unser M. (2017 Aug 1)

Compressed sensing for STEM tomography

ULTRAMICROSCOPY : 179 : 47-56 : DOI : 10.1016/j.ultramic.2017.04.003
Lu Zhou, Dapeng Zhang, Sabrina Hocine, Alessia Pilone, Sylvain Trépout, Sergio Marco, Christophe Thomas, Jia Guo, Min-Hui Li (2017 Jul 26)

Transition from smectic nanofibers to smectic vesicles in the self-assemblies of PEG-b-liquid crystal polycarbonates

Polymer Chemistry : 8 : 4776-4780 : DOI : 10.1039/C7PY01131H
Marie-Paule Teulade-Fichou, Joël Lefebvre, Corinne Guetta, Florent Poyer, Florence Mahuteau-Betzer (2017 Jul 13)

Copper-alkyne complexation is responsible for the Nucleolar Localisation of Quadruplex Nucleic Acid Drugs Labelled by Click Chemistry.

Angewandte Chemie (International ed. in English) : Online publication : DOI : 10.1002/anie.201703783
All publications