Modeling and Multimodal and Multiparametric Imaging

Our goal is to contribute, via integrated-imaging approaches and molecular modelling, to drug design and to the understanding of the effects and action mechanisms of diagnostic, therapeutic, and theranostic agents on cells, tissues and organisms.

 

Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).
Example of image processing and software development for chemical mapping: EFTEM-TomoJ user interface (left) and workflow including multivariate statistical analysis for signal-to-noise ratio improvement (right). From Messaoudi et al. 2013. Microsc. Microanal. 28:1-9).

For this purpose, we develop and optimize methods and tools (software, image acquisition procedures, preparation techniques and methods to improve sensitivity and resolution of imaging equipment) in the context of multimodal and/or multiscale imaging (including nano-SIMS, TEM-STEM-EFTEM, MRI) and molecular modelling (docking and dynamics). We mainly apply these developments to oncology and radio-biology thru close collaborations with several biology groups. We focus our activities on:

  1. the multiparametric/multiscale imaging developments to assess heterogeneity of healthy and cancer tissues characteristics
  2. the understanding of the effects of photon irradiation of photosensitizing molecules
  3. the characterization and tracking of radiosensitizing nanoparticles and drugs within cells
  4. the study of the bio-distribution and pharmacokinetic of drugs and chemical elements (I, P, Ca, Fe,….) in normal or pathogenic tissues and cells
  5. the search of small molecules with medicinal interest by computer-aided drug design
  6. the understanding at the atomic level of the mechanism of action of some proteins of biological interest by the use of normal mode analysis (NMA) and molecular dynamics (MD).

The developments and tools we design becomes accessible to academic users and corporations through the facilities depending on us (MRI, chemical mapping and tomography as part of the Cellular and Imaging facility of the Institut Curie) and through the Institut Carnot-Global Care.

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Key publications

Year of publication 2018

Partouche D., Turbant F., El Hamoui O., Campidelli C., Bombled M., Trépout S., Wien F., Arluison V. (2018 Dec 1)

Epigallocatechin Gallate Remodelling of Hfq Amyloid-Like Region Affects Escherichia coli Survival

Pathogens : 7 : 95 : DOI : 10.3390/pathogens7040095
Nathalie Fretellier, Agnès Granottier, Marlène Rasschaert, Anne-Laure Grindel, Fannie Baudimont, Philippe Robert, Jean-Marc Idée, Claire Corot (2018 Nov 6)

Does Age Interfere With Gadolinium Toxicity and Presence in Brain and Bone Tissues?: A Comparative Gadoterate Versus Gadodiamide Study in Juvenile and Adult Rats.

Investigative radiology : Publish Ahead of Print : DOI : 10.1097/RLI.0000000000000517
M Lupu, Ph Maillard, J Mispelter, F Poyer, C D Thomas (2018 Nov 1)

A glycoporphyrin story: from chemistry to PDT treatment of cancer mouse models.

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology : 17 : 1599-1611 : DOI : 10.1039/c8pp00123e
Tom Baladi, Jessy Aziz, Florent Dufour, Valentina Abet, Véronique Stoven, François Radvanyi, Florent Poyer, Ting-Di Wu, Jean-Luc Guerquin-Kern, Isabelle Bernard-Pierrot, Sergio Marco Garrido, Sandrine Piguel (2018 Nov 1)

Design, synthesis, biological evaluation and cellular imaging of imidazo[4,5-b]pyridine derivatives as potent and selective TAM inhibitors.

Bioorganic & medicinal chemistry : 26 : 5510-5530 : DOI : 10.1016/j.bmc.2018.09.031
Eloïse Bertiaux, Adeline Mallet, Cécile Fort, Thierry Blisnick, Serge Bonnefoy, Jamin Jung, Moara Lemos, Sergio Marco, Sue Vaughan, Sylvain Trépout, Jean-Yves Tinevez, Philippe Bastin (2018 Oct 3)

Bidirectional intraflagellar transport is restricted to two sets of microtubule doublets in the trypanosome flagellum.

The Journal of cell biology : 217 : 4284 : DOI : 10.1083/jcb.201805030
Philippe Robert, Thomas Frenzel, Cécile Factor, Gregor Jost, Marlène Rasschaert, Gunnar Schuetz, Nathalie Fretellier, Janina Boyken, Jean-Marc Idée, Hubertus Pietsch (2018 Sep 1)

Methodological Aspects for Preclinical Evaluation of Gadolinium Presence in Brain Tissue: Critical Appraisal and Suggestions for Harmonization-A Joint Initiative.

Investigative radiology : 53 : 499-517 : DOI : 10.1097/RLI.0000000000000467
All publications