UMR3664 – Nuclear Dynamics

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Teams in this unit investigate the mechanisms underlying the stability and the plasticity of genetic and epigenetic information in normal or pathological contexts such as cancer. Using complementarity approaches, we develop an integrated view of the functional organization of the genome at different scales: from the molecule to the cell to the organism.

Using several model organisms (Drosophila, Xenopus, mouse, yeast) and cell lines (human, rodents, insects …) we study fundamental processes of chromosome biology: DNA replication, segregation and repair, regulation of gene expression during development, cell cycle and in response to environmental stress

Together, these models are helping to decipher how DNA replication and repair, gene transcription and silencing  are modulated during development, cell division and in response to environmental stress

The main research themes of the unit include:

  • The roles of factors involved in chromatin dynamics, genome stability and repair
  • How functional domains of eukaryotic genomes are established and then maintained during development.
  • How epigenetic plasticity plays a part in controlling the polarity of the embryo.
  • How nuclear compartmentalisation and dynamics participate in regulating various functions of the genome.

 

Key publications

Year of publication 2016

Christèle Maison, Delphine Bailly, Jean-Pierre Quivy, Geneviève Almouzni (2016 Jul 19)

The methyltransferase Suv39h1 links the SUMO pathway to HP1α marking at pericentric heterochromatin.

Nature communications : 12224 : DOI : 10.1038/ncomms12224
Lisa Prendergast, Sebastian Müller, Yiwei Liu, Hongda Huang, Florent Dingli, Damarys Loew, Isabelle Vassias, Dinshaw J Patel, Kevin F Sullivan, Geneviève Almouzni (2016 Jun 11)

The CENP-T/-W complex is a binding partner of the histone chaperone FACT.

Genes & development : 1313-26 : DOI : 10.1101/gad.275073.115
A Coulon, D R Larson (2016 Jun 1)

Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory.

Methods in enzymology : 159-91 : DOI : 10.1016/bs.mie.2016.03.017

Year of publication 2015

Tineke L Lenstra, Antoine Coulon, Carson C Chow, Daniel R Larson (2015 Nov 10)

Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription.

Molecular cell : 597-610 : DOI : 10.1016/j.molcel.2015.09.028
Micol Guidi, Myriam Ruault, Martial Marbouty, Isabelle Loïodice, Axel Cournac, Cyrille Billaudeau, Antoine Hocher, Julien Mozziconacci, Romain Koszul, Angela Taddei (2015 Apr 2)

Spatial reorganization of telomeres in long-lived quiescent cells.

Genome biology : 206 : DOI : 10.1186/s13059-015-0766-2
Diana A Stavreva, Antoine Coulon, Songjoon Baek, Myong-Hee Sung, Sam John, Lenka Stixova, Martina Tesikova, Ofir Hakim, Tina Miranda, Mary Hawkins, John A Stamatoyannopoulos, Carson C Chow, Gordon L Hager (2015 Feb 14)

Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing.

Genome research : 845-57 : DOI : 10.1101/gr.184168.114