Non Coding RNA, Epigenetic and Genome Fluidity

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Antonin Morillon Non-coding RNA, epigenetics and genome fluidity Tel:

Our research interest is focused on non coding (nc)RNAs, representing the “dark matter” of the genome. In eukaryotes, ncRNAs have been shown to regulate gene expression, chromatin domains and genome stability. A growing number of evidence suggests that they play central roles on cancer development and cellular differentiation. Regulatory ncRNA can be classified in two categories depending on their size.

Short interfering (si)RNAs, also known to be part of the RNA interference pathway, have been extensively studied and control gene expression and chromosome segregation. Large (l)ncRNAs participate also in gene silencing and are key players in cell differentiation and development but, in contrast to siRNAs, their mode(s) of action remain poorly characterized. Our lab was one of the first to describe lncRNA-mediated epigenetic regulation controlling transposon proliferation and gene expression in the budding yeast providing powerful genetic and large scale tools to uncover their regulatory mechanisms in this classic model organism.

Morillon1

Since the beginning of our research project in 2005, we obtained two main results showing the existence of a trans-acting ncRNA controlling the Ty1 transposon in yeast (Figure 1). In addition, we provided evidence that cryptic transcription mediates the deposition of histone marks controlling inducible genes. Finally using RNA-seq technology, we recently defined an entire family of antisense regulatory ncRNA in yeast, that we called XUTs (Figure 2).

Our work shows that yeast is indeed an excellent organism to study regulatory ncRNA that are involved in chromatin regulation. Interestingly, the processing and the mode of action of the yeast large ncRNA implicate pathways important for genome integrity and cell development in mammalian cells, suggesting that their mechanisms might be conserved among the eukaryotic kingdom.

Our future aims are to extensively identify all the regulatory ncRNA in yeast and to further characterize their associated proteins to understand the mechanisms controlling histone modifications both at the euchromatin and heterochromatin domains. Our ongoing work will set up the fundamental basis for future studies in higher eukaryotes, especially during differentiation and cancer.

Key publications

Year of publication 2018

Antoine Hocher, Myriam Ruault, Petra Kaferle, Marc Descrimes, Mickaël Garnier, Antonin Morillon, Angela Taddei (2018 Oct 26)

Expanding heterochromatin reveals discrete subtelomeric domains delimited by chromatin landscape transitions.

Genome research : DOI : gr.236554.118
Alculumbre SG, Saint-André V, Di Domizio J, Vargas P, Sirven P, Bost P, Maurin M, Maiuri P, Wery M, Roman MS, Savey L, Touzot M, Terrier B, Saadoun D, Conrad C, Gilliet M, Morillon A, Soumelis V (2018 Jan 1)

Diversification of human plasmacytoid predendritic cells in response to a single stimulus

Nature Immunology : 19(1) : 63-75 : DOI : 10.1038/s41590-017-0012-z

Year of publication 2017

Wery M, Gautier C, Descrimes M, Yoda M, Vennin-Rendos H, Migeot V, Gautheret D, Hermand D, Morillon A (2017 Nov 7)

Native Elongating Transcript Sequencing reveals global anti-correlation between sense and antisense nascent transcription in fission yeast

RNA : DOI : 10.1261/rna.063446.117
Jarroux J, Morillon A, Pinskaya M. (2017 Oct 1)

History, Discovery, and Classification of lncRNAs

Adv Exp Med BiolLong non coding RNA biology : 1008 : 1-46 : DOI : 10.1007/978-981-10-5203-3_1

Year of publication 2016

Maxime Wery, Marc Descrimes, Nicolas Vogt, Anne-Sophie Dallongeville, Daniel Gautheret, Antonin Morillon (2016 Jan 26)

Nonsense-Mediated Decay Restricts LncRNA Levels in Yeast Unless Blocked by Double-Stranded RNA Structure.

Molecular cell : 379-92 : DOI : 10.1016/j.molcel.2015.12.020

Year of publication 2015

Marta Kwapisz, Myriam Ruault, Erwin van Dijk, Stephanie Gourvennec, Marc Descrimes, Angela Taddei and Antonin Morillon (2015 Jul 3)

Expression of Subtelomeric lncRNAs Links Telomeres Dynamics to RNA Decay in S. cerevisiae

Non-coding RNA : 1 : 94-126 : DOI : 10.3390/ncrna1020094
All publications