UMR3244 – Dynamics of Genetic Information

Teams in this unit aim at elucidating the regulation of mechanisms that are crucial to the maintenance of genome integrity, like DNA replication, repair and recombination, as well as the role of cell cycle checkpoints and non-coding RNAs in genome and epigenome maintenance.

Experimental models include yeast, mammalian and human cell lines, mutant mouse models and tumour samples. A large variety of methods is used including genetic, molecular and cytogenetic techniques, fluorescence in situ hybridisation and DNA ‘combing’, as well as high throughput sequencing approaches such as DNA-seq for mutation landscape analyses, RNA-seq for transcriptome analyses and ChIP-seq mapping of chromatin-associated proteins and their genome-wide modulation in response to DNA damages.

 

Key publications

Year of publication 2019

Olivier Brison, Sami El-Hilali, Dana Azar, Stéphane Koundrioukoff, Mélanie Schmidt, Viola Nähse, Yan Jaszczyszyn, Anne-Marie Lachages, Bernard Dutrillaux, Claude Thermes, Michelle Debatisse, Chun-Long Chen (2019 Dec 15)

Transcription-mediated organization of the replication initiation program across large genes sets common fragile sites genome-wide.

Nature communications : 5693 : DOI : 10.1038/s41467-019-13674-5
Mireille Bétermier, Valérie Borde, Jean-Pierre de Villartay (2019 Dec 11)

Coupling DNA Damage and Repair: an Essential Safeguard during Programmed DNA Double-Strand Breaks?

Trends in cell biology : DOI : S0962-8924(19)30201-6
(2019 Nov 15)

Reference-free transcriptome exploration reveals novel RNAs for prostate cancer diagnosis.

Life Sci Alliance : DOI : 10.26508/lsa.201900449
Emilia Puig Lombardi, Allyson Holmes, Daniela Verga, Marie-Paule Teulade-Fichou, Alain Nicolas, Arturo Londoño-Vallejo (2019 Jul 9)

Thermodynamically stable and genetically unstable G-quadruplexes are depleted in genomes across species.

Nucleic acids research : 47 : 6098-6113 : DOI : 10.1093/nar/gkz463
Kevin Grosselin, Adeline Durand, Justine Marsolier, Adeline Poitou, Elisabetta Marangoni, Fariba Nemati, Ahmed Dahmani, Sonia Lameiras, Fabien Reyal, Olivia Frenoy, Yannick Pousse, Marcel Reichen, Adam Woolfe, Colin Brenan, Andrew D. Griffiths*, Céline Vallot* & Annabelle Gérard* (2019 May 31)

High-throughput single-cell ChIP-seq identifies heterogeneity of chromatin states in breast cancer

Nature Genetics : 1060–1066 : DOI : 10.1038/s41588-019-0424-9
(2019 May 1)

Long non-coding RNAs: towards urinary diagnosis for prostate cancer

Bulletin de l'Académie Nationale de Médecine : DOI : https://doi.org/10.1016/j.banm.2018.03.001