UMR3244 – Dynamics of Genetic Information

Teams in this unit aim at elucidating the regulation of mechanisms that are crucial to the maintenance of genome integrity, like DNA replication, repair and recombination, as well as the role of cell cycle checkpoints and non-coding RNAs in genome and epigenome maintenance.

Experimental models include yeast, mammalian cell lines, mutant mouse models and tumour samples. A large variety of methods is used including genetic, molecular and cytogenetic techniques, fluorescence in situ hybridisation and DNA ‘combing’, as well as high throughput sequencing approaches such as DNA-seq for mutation landscape analyses, RNA-seq for transcriptome analyses and ChIP-seq mapping of chromatin-associated proteins and their genome-wide modulation in response to DNA damages.

 

Key publications

Year of publication 2021

Marsolier, Justine Prompsy, Pacôme Durand, Adeline Lyne, Anne-Marie Landragin, Camille Trouchet, Amandine Bento, Sabrina Tenreira Eisele, Almut Foulon, Sophie Baudre, Léa Grosselin, Kevin Bohec, Mylène Baulande, Sylvain Dahmani, Ahmed Sourd, Laura Letouzé, Eric Marangoni, Elisabetta Perié, Leïla Vallot, Céline (2021 Jan 4)

H3K27me3 is a determinant of chemotolerance in triple-negative breast cancer

bioRxivbioRxiv : DOI : 10.1101/2021.01.04.423386

Year of publication 2020

Aurore Sanchez, Céline Adam, Felix Rauh, Yann Duroc, Lepakshi Ranjha, Bérangère Lombard, Xiaojing Mu, Mélody Wintrebert, Damarys Loew, Alba Guarné, Stefano Gnan, Chun-Long Chen, Scott Keeney, Petr Cejka, Raphaël Guérois, Franz Klein, Jean-Baptiste Charbonnier, Valérie Borde (2020 Nov 17)

Exo1 recruits Cdc5 polo kinase to MutLγ to ensure efficient meiotic crossover formation.

Proceedings of the National Academy of Sciences of the United States of America : DOI : 202013012
Pacôme Prompsy, Pia Kirchmeier, Justine Marsolier, Marc Deloger, Nicolas Servant, Céline Vallot (2020 Nov 12)

Interactive analysis of single-cell epigenomic landscapes with ChromSCape.

Nature communications : 5702 : DOI : 10.1038/s41467-020-19542-x
Gnan Stefano, Liu Yaqun, Spagnuolo Manuela, Chen Chun-Long (2020 Aug 31)

The impact of transcription-mediated replication stress on genome instability and human disease

Genome Instability & Disease : 1 : 207-234 : DOI : 10.1007/s42764-020-00021-y
Felix Raimundo, Celine Vallot, Jean-Philippe Vert (2020 Aug 25)

Tuning parameters of dimensionality reduction methods for single-cell RNA-seq analysis.

Genome biology : 212 : DOI : 10.1186/s13059-020-02128-7
Elda Cannavo, Aurore Sanchez, Roopesh Anand, Lepakshi Ranjha, Jannik Hugener, Céline Adam, Ananya Acharya, Nicolas Weyland, Xavier Aran-Guiu, Jean-Baptiste Charbonnier, Eva R Hoffmann, Valérie Borde, Joao Matos, Petr Cejka (2020 Aug 21)

Regulation of the MLH1-MLH3 endonuclease in meiosis.

Nature : DOI : 10.1038/s41586-020-2592-2