UMR3244 – Dynamics of Genetic Information

Teams in this unit aim at elucidating the regulation of mechanisms that are crucial to the maintenance of genome integrity, like DNA replication, repair and recombination, as well as the role of cell cycle checkpoints and non-coding RNAs in genome and epigenome maintenance.

Experimental models include yeast, mammalian cell lines, mutant mouse models and tumour samples. A large variety of methods is used including genetic, molecular and cytogenetic techniques, fluorescence in situ hybridisation and DNA ‘combing’, as well as high throughput sequencing approaches such as DNA-seq for mutation landscape analyses, RNA-seq for transcriptome analyses and ChIP-seq mapping of chromatin-associated proteins and their genome-wide modulation in response to DNA damages.

 

Key publications

Year of publication 2021

Weitao Wang, Kyle N Klein, Karel Proesmans, Hongbo Yang, Claire Marchal, Xiaopeng Zhu, Tyler Borrman, Alex Hastie, Zhiping Weng, John Bechhoefer, Chun-Long Chen, David M Gilbert, Nicholas Rhind (2021 Jun 22)

Genome-wide mapping of human DNA replication by optical replication mapping supports a stochastic model of eukaryotic replication.

Molecular cell : DOI : S1097-2765(21)00408-1
Jingqi Dai, Aurore Sanchez, Céline Adam, Lepakshi Ranjha, Giordano Reginato, Pierre Chervy, Carine Tellier-Lebegue, Jessica Andreani, Raphaël Guérois, Virginie Ropars, Marie-Hélène Le Du, Laurent Maloisel, Emmanuelle Martini, Pierre Legrand, Aurélien Thureau, Petr Cejka, Valérie Borde, Jean-Baptiste Charbonnier (2021 Jun 5)

Molecular basis of the dual role of the Mlh1-Mlh3 endonuclease in MMR and in meiotic crossover formation.

Proceedings of the National Academy of Sciences of the United States of America : DOI : e2022704118
Samar Ali, Emilia Puig Lombardi, Deepanjan Ghosh, Tao Jia, Géraldine Vitry, Lina Saker, Joël Poupon, Marie-Paule Teulade-Fichou, Alain Nicolas, Arturo Londono-Vallejo, Sophie Bombard (2021 May 22)

Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage.

Metallomics : integrated biometal science : 13 : mfab029 : DOI : 10.1093/mtomcs/mfab029
Julien Jarroux, Dominika Foretek, Claire Bertrand, Marc Gabriel, Ugo Szachnowski, Zohra Saci, Shuling Guo, Arturo Londoño-Vallejo, Marina Pinskaya, Antonin Morillon (2021 May 7)

HOTAIR lncRNA promotes epithelial-mesenchymal transition by redistributing LSD1 at regulatory chromatin regions.

EMBO reports : e50193 : DOI : 10.15252/embr.202050193
Dipti Vinayak Vernekar, Giordano Reginato, Céline Adam, Lepakshi Ranjha, Florent Dingli, Marie-Claude Marsolier, Damarys Loew, Raphaël Guérois, Bertrand Llorente, Petr Cejka, Valérie Borde (2021 Apr 6)

The Pif1 helicase is actively inhibited during meiotic recombination which restrains gene conversion tract length.

Nucleic acids research : DOI : gkab232
Marsolier, Justine Prompsy, Pacôme Durand, Adeline Lyne, Anne-Marie Landragin, Camille Trouchet, Amandine Bento, Sabrina Tenreira Eisele, Almut Foulon, Sophie Baudre, Léa Grosselin, Kevin Bohec, Mylène Baulande, Sylvain Dahmani, Ahmed Sourd, Laura Letouzé, Eric Marangoni, Elisabetta Perié, Leïla Vallot, Céline (2021 Jan 4)

H3K27me3 is a determinant of chemotolerance in triple-negative breast cancer

bioRxivbioRxiv : DOI : 10.1101/2021.01.04.423386