Polarity Division Morphogenesis

Yohanns Bellaiche

Yohanns Bellaïche Team Leader Tel:

Cell polarity is fundamental to the development of multi-cellular organisms, playing a central role in proliferation, cell fate diversification and tissue homeostasis or morphogenesis. The mechanisms controlling cell polarization are conserved, and the Drosophila fruit fly model has played a leading role in helping our understanding of the mechanisms controlling cell polarization during development.

Our interdisciplinary research group, composed of biologists and physicists, studies the development of epithelial tissue and the specification of distinct cell fates upon asymmetric cell division in Drosophila. Our work on asymmetric cell division has unravelled mechanisms regulating cortical cell polarity and mitotic spindle orientation. This work has also provided an experimental and conceptual foundation with which to study epithelial tissue development. In the next five years, we aim to extend our study of epithelial tissue, using the following approaches:

  1. Modulating cell polarization at the subcellular level. To complement the panoply of Drosophila genetic tools, we aim to manipulate cell polarity at the sub-cellular level and in a temporally control manner. To this end we will implement optogenetic control of polarization to finely modify cell polarization and therefore decipher its role in cell shape and organization. 
  1. Dissecting the molecular pathways controlling epithelial polarization. We are coupling genetic tools to state of the art time-lapse microscopy to dissect the mechanisms of cortical cell polarization and mitotic spindle orientation. Our expertise allows us to tackle two central questions in the field of epithelial tissue: how is the mitotic spindle oriented in epithelial tissue? And how is cell polarity maintained and transmitted through cell division?
  1. Linking the cellular scale to the tissue scale. To integrate our knowledge of the role of cell polarization and mitotic spindle orientation from the cell level to the tissue level, we are implementing physical methodologies to provide a quantitative readout of how the regulation of cell shape, of cell division orientation and of cell movement impact on the morphogenesis and homeostasis of an epithelial tissue.

Using both conventional and innovative methodologies, our goals over the next years are to better understand how molecules and protein complexes are moved and activated at different locations within the cell and how cell polarization impacts on epithelial tissue growth and morphogenesis. The work will rely to a great extent on the interdisciplinary nature of the team. Furthermore this interdisciplinarity underlies our long-term perspective to integrate gene regulation and cell mechanics in order to understand tissue development. Since the mechanisms underlying cell polarization are conserved throughout evolution, the findings should improve our understanding of these processes not only in Drosophila, but in all animals.

figure 1 : Cytocinèse d’une cellule épithéliale (Herszterg et al., Dev Cell 2013)
figure 1 : Cytokinesis of an epithelial cell (Herszterg et al., Dev Cell 2013)

 

figure 2 : Prolifération au sein de l’épithélium du thorax (Bosveled et al., Science 2012).
figure 2 : Proliferation in the epithelium of the dorsal thorax (Bosveled et al., Science 2012).

 

 

Key publications

Year of publication 2016

Floris Bosveld, Olga Markova, Boris Guirao, Charlotte Martin, Zhimin Wang, Anaëlle Pierre, Maria Balakireva, Isabelle Gaugue, Anna Ainslie, Nicolas Christophorou, David K. Lubensky, Nicolas Minc & Yohanns Bellaïche (2016 Feb 25)

Epithelial tricellular junctions act as interphase cell shape sensors to orient mitosis

Nature : 530 : 495–498 : DOI : 10.1038/nature16970

Year of publication 2015

Floris Bosveld, Boris Guirao, Zhimin Wang, Mathieu Rivière, Isabelle Bonnet, François Graner, Yohanns Bellaïche (2015 Jul 5)

Modulation of junction tension by tumor suppressors and proto-oncogenes regulates cell-cell contacts.

Development (Cambridge, England) : 623-34 : DOI : 10.1242/dev.127993
Boris Guirao, Stéphane U Rigaud, Floris Bosveld, Anaïs Bailles, Jesús López-Gay, Shuji Ishihara, Kaoru Sugimura, François Graner, Yohanns Bellaïche (2015 May 6)

Unified quantitative characterization of epithelial tissue development.

eLife : DOI : 10.7554/eLife.08519

Year of publication 2012

Carl-Philipp Heisenberg, Yohanns Bellaïche (2012 Dec 22)

Forces in tissue morphogenesis and patterning.

Cell : 948-62 : DOI : 10.1016/j.cell.2013.05.008
Sophie Herszterg, Andrea Leibfried, Floris Bosveld, Charlotte Martin, Yohanns Bellaiche (2012 Sep 11)

Interplay between the dividing cell and its neighbors regulates adherens junction formation during cytokinesis in epithelial tissue.

Developmental cell : 256-70 : DOI : 10.1016/j.devcel.2012.11.019
Pierre-Luc Bardet, Boris Guirao, Camille Paoletti, Fanny Serman, Valentine Léopold, Floris Bosveld, Yûki Goya, Vincent Mirouse, François Graner, Yohanns Bellaïche (2012 Aug 4)

PTEN controls junction lengthening and stability during cell rearrangement in epithelial tissue.

Developmental cell : 534-46 : DOI : 10.1016/j.devcel.2013.04.020
All publications