The post-doctoral researcher will be part of the team “Probes and Drugs of Nucleic acids Secondary Structures” led by Dr. Teulade-Fichou (https://science.curie.fr/recherche/equipe-teulade-fichou/) in the laboratory “Chemistry and Modelling for the Biology of Cancer” (CMBC) located at Institut Curie, Orsay. He/she will develop a project to study the interactions between proteins and DNA or RNA secondary structures called quadruplexes (G4). A first part of the project will be devoted to biochemical and biophysical studies of the G4/protein interactions in vitro, a second part will be devoted to the exploration of G4/protein interactions in various cellular contexts, in particular in Epstein-Barr virus (EBV)-infected cancers cells. The details of the project will be directly discussed with the applicants. He/she will work under the direction of Dr. Marie-Paule Teulade-Fichou (CNRS Research Director, team leader and head of the CMBC Laboratory) in collaboration with other team members as part of the project supported by the Fondation pour la Recherche Médicale (FRM) grant, in association with the teams of Anton Granzhan (junior team, CMBC / Institut Curie), Marc Blondel (INSERM / University of Western Brittany) and Robin Farhaeus (INSERM, St. Louis Hospital, Paris).
Activities: The post-doctoral fellow will be in charge of developing his/her project. He/she will manage the design, plan, execute and interpret various experiments. He/she must master the associated literature, must be able to communicate his/her results to the team, the institute and the collaborators. He/she must be able to work independently as well as interact with other team members and collaborators.
Skills: We are looking for a highly motivated and dynamic scientist. The candidate must hold a Ph.D. degree in Biochemistry, Biophysics, or Molecular and Cellular Biology, and have an excellent knowledge of nucleic acid structures and protein/nucleic acid interactions. Hands-on experience with state-of-the-art biochemical and biophysical techniques for studies of protein/nucleic acid interactions in vitro and in cellulo as well as cellular imaging are required. The candidate must be an author or co-author of at least one publication related to the subject or skills required for the project. Applicants must be able to fluently communicate in English (oral and written skills).
Work context: The research activity of the team at the interface between chemistry and biology is mainly focused on targeting and imaging of nucleic acids, with a particular emphasis on non-canonical G-quadruplex (G4) DNA/RNA structures. The overall goals are i) to develop strategies and probes to image and map G4-interactive compounds distribution in cells and at the genome-wide level and ii) to explore the anticancer therapeutic potential of G4-interactive compounds. The team is multidisciplinary with complementary skills and expertise area spanning chemical biology and biochemistry to cellular biology and clinical research. The candidate will benefit from the scientific environment of the Institut Curie (both in Orsay and in Paris) and will have access to the numerous technical platforms (chemical library, protein production, imaging, NGS).
References related to the project.
Lista, M. J.; Martins, R. P.; Billant, O.; Contesse, M.-A.; Findakly, S.; Pochard, P.; Daskalogianni, C.; Beauvineau, C.; Guetta, C.; Jamin, C.; Teulade-Fichou, M.-P.; Fåhraeus, R.; Voisset, C.; Blondel, M. Nucleolin directly mediates Epstein-Barr virus immune evasion through binding to G-quadruplexes of EBNA1 mRNA. Nat. Commun. 2017, 8, 16043.
Reznichenko, O.; Quillévéré, A.; Martins, R. P.; Loaëc, N.; Kang, H.; Lista, M. J.; Beauvineau, C.; González-García, J.; Guillot, R.; Voisset, C.; Daskalogianni, C.; Fåhraeus, R.; Teulade-Fichou, M.-P.; Blondel, M.; Granzhan, A. Novel cationic bis(acylhydrazones) as modulators of Epstein-Barr virus immune evasion acting through disruption of interaction between nucleolin and G-quadruplexes of EBNA1 mRNA. Eur. J. Med. Chem. 2019, 178, 13–29.
Saha, A.; Duchambon, P.; Masson, V.; Loew, D.; Bombard, S.; Teulade-Fichou, M.-P. Nucleolin Discriminates Drastically between Long-Loop and Short-Loop Quadruplexes. Biochemistry 2020, 59, 1261–1272.
Contract duration: 18 months
Expected date of employment: mid to end of October 2020
Remuneration: gross monthly salary around 2700 €
Applications and inquires: applications (CV, cover letter, contact details of 2 referees) should be submitted to Dr. Marie-Paule Teulade-Fichou () by September 30th, 2020. Interviews will be held by end of September.