Synthetic Genetic Array (SGA)

Activity

Synthetic Genetic Array (SGA) is a high throughput approach allowing the systematic generation of double mutants. Using a query strain, carrying a mutation of interest that is then crossed with a library of mutants, one can obtain double mutants and score for synthetic interactions. Such screens have been used extensively over the years to decipher the complexities of genetic networks and pathways in yeast. A fully equipped laboratory dedicated to SGA technique has been established at Institut Curie in 2012 and is available to all interested yeast and non-yeast research groups.

Aims

  • Identifying genetic interactions between genes involved in individual pathways
  • Studying gene or protein functions
  • Elucidating mechanism of action of drugs

Networking

  • Manolis  Papamichos-Chronakis,  Institut Curie, Paris, France
  • Angela Taddei, Institut Curie, Paris, France
  • Antonin Morillon, Institut Curie, Paris, France
  • Stéphane Marcand, CEA, Fontenay-Aux-Roses
  • Philippe Pasero, IGH, Montpellier, France
  • Jorrit Enserink, Rikshospitalet University Hospital, Oslo, Norway

Services

Agar-based assays:

  • chemical genomics
  • synthetic genetic array
  • E-MAP

Liquid-based assays:

  • growth curves
  • colorimetric assays

Equipment

Robotic manipulator BM3-BC (S&P Robotics, Inc.):

  • 96, 384 and 1536-format replicating heads
  • 96-format re-arraying head
  • capacity 48 plates
  • integrated camera

Spectrometer Eon + plate handler BioStacker3 (BioTek):

  • 200 to 999 nm wavelength range
  • Different modes of shaking
  • Incubation chamber

S.cerevisiae collections:

  • Yeast Knockout Collection
  • Temperature-sensitive mutant collection
  • Decreased Abundance by mRNA Perturbation (DAmP) collection
  • Yeast Synthetic Histone Collection
  • GFP-tagged ORFs
  • VN-Fusion Library

Training

SGA platform does not offer any training.