Protein Expression and Purification Core Facility

Activity

The Protein Production and Purification Facility is a core service and resource to overcome the major bottleneck in recombinant protein expression and purification. Mainly we provide advice and active support for researchers scientific community in the use of the procaryotic and eukaryotic expression system for recombinant protein production.

The facility offers equipment and materials required for the expression of protein in bacterial, Yeast, insect (baculovirus) and mammalian cell. We can execute the complete cloning, expression, scale-up and purification process or specific tasks within the project.

We have optimized different expressions vectors in combination with various bacterial strains, yeast strain, insect strain and mammalian cell for specific expression of different types of proteins.

ProteineRecombinante1

 

Mission

The Protein Expression Facility is a fee-for platform dedicated to providing technical assistance in the following areas:

  • Advices and training
  • Recombinant DNA engineering
  • Recombinant protein expression in bacteria and yeast
  • Recombinant protein expression via baculovirus expression systems (BVES)
  • Recombinant protein expression in Mammalian cells
  • Protein purification
  • Polyclonals antibodies production

ProteineRecombinante2

ProteineRecombinante3

Services

The platform possesses expertise to produce and to purify any type of protein. For each project we used the following flowchart:

  • Primer design and expression vector cloning
  • Strains development
  • Expression screening
  • Production and purification

Other Services

  • Preparation and distribution of recombinant expression vector
  • New Vector development to provide state-of-the-art expression technologies
  • Maintenance of frozen stocks of recombinant baculovirus
  • Protein purification technology development
  • Development of recombinant protein and molecular biology enzymes catalogue

ProteineRecombinante4

Equipments

Fermenter for cell culture and protein production
Chromatography system: for protein purification
Cell Disruption: proteins extraction
Insect cell culture for protein production
Hybridoma system production
Akta flux for sample concentration

Network and collaboration

P4EU: (Protein Production and Purification Partnership in Europe) network initiative

Collaboration with Recombinant Platform facility of Institut Pasteur

Training

The facility functions as a training centre for students and scientists in gene cloning and Recombinant protein production.

Key publications

Year of publication 2016

Catharina von Nicolai, Åsa Ehlén, Charlotte Martin, Xiaodong Zhang, Aura Carreira (2016 Sep 15)

A second DNA binding site in human BRCA2 promotes homologous recombination.

Nature communications : 12813 : DOI : 10.1038/ncomms12813

Year of publication 2015

Serena Sanulli, Neil Justin, Aurélie Teissandier, Katia Ancelin, Manuela Portoso, Matthieu Caron, Audrey Michaud, Berangère Lombard, Simao T da Rocha, John Offer, Damarys Loew, Nicolas Servant, Michel Wassef, Fabienne Burlina, Steve J Gamblin, Edith Heard, Raphaël Margueron (2015 Mar 5)

Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation.

Molecular cell : 769-83 : DOI : 10.1016/j.molcel.2014.12.020

Year of publication 2014

Laura Picas, Julien Viaud, Kristine Schauer, Stefano Vanni, Karim Hnia, Vincent Fraisier, Aurélien Roux, Patricia Bassereau, Frédérique Gaits-Iacovoni, Bernard Payrastre, Jocelyn Laporte, Jean-Baptiste Manneville, Bruno Goud (2014 Dec 9)

BIN1/​M-Amphiphysin2 induces clustering of phosphoinositides to recruit its downstream partner dynamin

Nature Communications5 : DOI : 10.1038/ncomms6647
Abdeslam Et Taouil, Emilie Brun, Patricia Duchambon, Yves Blouquit, Manon Gilles, Emmanuel Maisonhaute, Cécile Sicard-Roselli (2014 Oct 14)

How protein structure affects redox reactivity: example of Human centrin 2.

Physical chemistry chemical physics : PCCP : 16 : 24493-24498 : DOI : 10.1039/c4cp03536d

Year of publication 2013

Xavier Lahaye, Takeshi Satoh, Matteo Gentili, Silvia Cerboni, Cécile Conrad, Ilse Hurbain, Ahmed El Marjou, Christine Lacabaratz, Jean-Daniel Lelièvre, Nicolas Manel (2013 Sep 6)

The capsids of HIV-1 and HIV-2 determine immune detection of the viral cDNA by the innate sensor cGAS in dendritic cells.

Immunity : 1132-42 : DOI : 10.1016/j.immuni.2013.11.002
Baligh Miladi, Cyrine Dridi, Ahmed El Marjou, Guilhem Boeuf, Hassib Bouallagui, Florence Dufour, Patrick Di Martino, Abdellatif Elm'selmi (2013 Jun 20)

An improved strategy for easy process monitoring and advanced purification of recombinant proteins.

Molecular biotechnology : 227-35 : DOI : 10.1007/s12033-013-9673-5
All publications