Cell & Tissue Imaging Platform

Chemical Mapping

 

Ultra-structural analysis of biological samples can be of great interest when combined with chemical mapping because it provides qualitative and semiquantitative information on the distribution of chemical elements on a given sample. This can be approached by secondary ion mass spectroscopy imaging, and by transmission electron microscopy in scanning (Z-contrast) end energy loss modes (Electron Energy Filtered TEM or EFTEM). Combine these two last modes with electron tomography, for biological samples, constitutes nowadays a technological defy that we have approached together with the correlation of SIMS data with chemical mapping by TEM.

Combining SIMS and EFTEM chemical mapping has allowed the biologists to validate hypothesis such as the role of ferritin in the survival of dopaminegic neurons in order to better understand the process of neuronal death implicated in neurodegenerative diseases such as Parkinson’s disease. The figure represents the different analysis done in SIMS and TEM chemical mapping in the same region. Iron granules observed by SIMS (colored at the left) corresponds to the aggregation or iron particles of about 5 nm as determined by EFTEM (right). (Funding ANR *****)
Combining SIMS and EFTEM chemical mapping has allowed the biologists to validate hypothesis such as the role of ferritin in the survival of dopaminegic neurons in order to better understand the process of neuronal death implicated in neurodegenerative diseases such as Parkinson’s disease. The figure represents the different analysis done in SIMS and TEM chemical mapping in the same region. Iron granules observed by SIMS (colored at the left) corresponds to the aggregation or iron particles of about 5 nm as determined by EFTEM (right). (Funding ANR *****)